Effect of simvastatin on rat femoral fracture healing.docVIP

 PAGE \* MERGEFORMAT 3 Effect of simvastatin on rat femoral fracture healing Author: Liang Chun-yu, Chang Liu, Zhaowen Guo, Cheng patriotic, Wang Lina [Keywords:] Sim valve [Keywords:] rat; bone mineral density; Bone histomorphometry; fracture healing; statins 0 Introduction Statin drugs are currently used to reduce cholesterol, the clinical prevention of cardiovascular disease, commonly used drugs, their mechanism of action is to inhibit HMG-CoA reductase A, thereby reducing hepatic cholesterol synthesis. Mundy et al [1] study is the first one shows that statins can activate osteoblasts, promote bone anabolic effect, this discovery for clinical treatment of osteoporosis and fracture healing provides new ideas, become the new drug for the study of bone metabolism in a hot issue. In order to further to explore the role of the drug into the bone and its mechanism, we have broken through the establishment of rat model [2] method, carried out the impact of drugs on fracture healing in rats preliminary experiments. 1 Materials and methods 1.1 Preparation of Animal Model and Grouping 12-week-old female SD rats were healthy 20, body mass (238 ± 22) g. to 10 g / L sodium pentobarbital by 40 mg / kg intraperitoneal injection of anesthesia, anesthesia after the success of the rat supine on the operating table, conventional to the hair, skin preparation, iodophor disinfection, in the middle of the right lateral thigh to take longitudinal skin incision, about 2 cm, along the vastus lateralis into the gap separating blunt up femoral shaft, a fixed femur, in the backbone of the 1 / 3 with the wire saw sawing into a transverse fracture, this process, attention to protecting the sciatic nerve and muscle tissue, and then, to a diameter of about 1.5 mm retrograde penetration of the Kirschner wire fracture of the proximal and from the posterior femoral greater trochanter falling, and then reset the fracture fracture of his distal anterograde penetration marrow, saline was