Effect of Simvastatin on PTHrP-induced osteoclastic bone resorption and mouse calvaria bone metabolism in.docVIP

 PAGE \* MERGEFORMAT 20 Effect of Simvastatin on PTHrP-induced osteoclastic bone resorption and mouse calvaria bone metabolism in Author: Huang Lu Yu, Yun-Yu Hu, TAO people, Bilong 【Key Words】 Simvastatin Abstract: [Objective] To investigate the simvastatin on the in vitro parathyroid hormone-related peptide (PTHrP)-induced osteoclastic bone resorption in mice the function of bone metabolism in mice and its effects. [Methods] PTHrP-induced osteoclast cell culture of mouse bone marrow cells and mouse calvarial culture system to detect the role of simvastatin after 8 d osteoclastic bone resorption, and calcium changes in the supernatant; test in mice calvaria bone alkaline phosphatase and calcium content of the supernatant, histological observation of morphological changes in mice calvaria bone. [Results] simvastatin could significantly inhibit the in vitro PTHrP induced osteoclast formation and bone resorption of calcium release supernatant, simvastatin can enhance mouse calvaria in vitro culture supernatant of bone alkaline phosphatase activity, histological observation of mice calvaria simvastatin increased bone mineralization. [Conclusion] simvastatin in vitro not only in mice calvaria can promote the osteogenic activity of bone, and can inhibit PTHrP-induced mice, osteoclastic bone resorption function, bone resorption disease prevention and treatment has an important role. Keywords: simvastatin; broken bone; bone resorption; parathyroid hormone-related peptide (PTHrP) Abstract: [Objective] To study the effect of simvastatin in the osteoclastic resorption stimulated by PTHrP and murine bone anabolism in vitro [Method] The bone resorption activities of the osteoclast stimulated by PTHrP were evaluated after treatment with simvastatin for 8 days in vitro; the concentration of Ca2 in the supernatant was also detected by atomic absorption spectrometerThe concentration of ALP and Ca2 of the supematant in murine calvarial organ culture