Effect of rosiglitazone on high glucose induced proliferation of vascular smooth muscle cells of inflammation and.docVIP

 PAGE \* MERGEFORMAT 18 Effect of rosiglitazone on high glucose induced proliferation of vascular smooth muscle cells of inflammation and Of: Zhao Zhansheng Liangjiang Yan Xie Yun Wang Mian Dengyong Gui Zhou Bin Su Cong Wang Ruiying Zhang Lihui Yao Yuxia dual wins [Abstract] Objective To study rosiglitazone (RSG) incubated under high glucose on vascular smooth muscle cells (VSMCs) inflammation and apoptosis and its possible molecular mechanism. Methods in different concentrations of glucose and rosiglitazone alone or co-incubated with rat aortic smooth muscle cells, ELISA was used to detect the medium of monocyte chemoattractant protein 1 (MCP 1) levels, using flow cytometry VSMCs apoptosis rate and Bcl xl, Bcl 2 protein expression , Western blot of cytosolic VSMCs Bcl xl expression and NF Bp65 and IB @ expression. the result of high glucose concentrations (11.2,22.5 mmol / L) in the culture supernatant significantly increased the concentration of MCP 1, and promote the proliferation of VSMCs, inhibition of apoptosis, increased Bcl xl, Bcl 2 protein expression, while the NF B p65 expression was increased, IB @ expression decreased, 30 and 100 mol / L RSG VSMCs in a concentration dependent manner to reduce the secretion of MCP 1, under the effect of high glucose culture (11.2,22.5 mmol / L) VSMCs Bcl xl, Bcl 2 expression and promote apoptosis, reduced expression of NF Bp65, promoting IB @ expression. RSG antagonist GW9662 (10 mol / L) pretreatment partially antagonized the effect of RSG. Conclusion RSG NF B pathway may be through regulation, reduce the secretion of inflammatory cytokines MCP 1, reduced Bcl xl, Bcl 2 expression, thereby inhibiting high glucose in VSMCs cultured in glucose and promote the apoptosis of inflammatory response to blood vessels in type 2 diabetic lesions play an important role in the prevention and treatment. [Keywords:] rosiglitazone, high glucose, proliferation, inflammation, vascular smooth muscle cells [Abstract] Obj