Elderly patients with Acinetobacter baumannii isolates in 16S rRNA methylase genes found in a new subtype of.docVIP

 PAGE \* MERGEFORMAT 5 Elderly patients with Acinetobacter baumannii isolates in 16S rRNA methylase genes found in a new subtype of Author: Zhu Jianming Jiang Ru-Jin Wu Zu-huang Mi Recreation [Abstract] Objective To understand Acinetobacter baumannii isolates in elderly patients with 16S rRNA methylase, and aminoglycoside-modifying enzyme genotypes. Methods PCR detection of 20 elderly patients with Acinetobacter baumannii isolates from 12 kinds of 16S rRNA methylase, and aminoglycoside-modifying enzyme genes. Results 7 detected in 16S rRNA methylase genes (armA), 19 Zhu detection of aminoglycoside-modifying enzyme genes [including aac (3)  I-positive rate of 95%, ant (3 amp;quot;)  I was 95% , aac (3)  II is 40%, aac (6 ‘)  Ib 15%)]. 6, the measured strains armA gene sequences translated into amino acid sequence and the United States nucleic acid library (GenBank) has been logged armA amino acids different from as a new subtype. Conclusions In this study of Acinetobacter baumannii isolates from 95% in elderly patients carrying 16S rRNA methylase, and aminoglycoside-modifying enzyme genes. [Keywords:] Acinetobacter baumannii; 16S rRNA methylase; aminoglycoside-modifying enzymes ABSTRACT Objective To investigate the genotype of 16S rRNA methylase and aminoglycoside  modifying enzymes in Acinetobacter baumannii isolates in the elderly patients. Methods The genes of 12 kinds 16S rRNA methylase and aminoglycoside  modifying enzymes were detected by polymerase chain reaction (PCR). Results In 20 strains, 7 strains carried 16S rRNA methylase gene (armA); 19 strains were positive for the genes of aminoglycoside  modifying enzymes [the positive rates of aac (3)  I, ant (3 amp;quot;)  I, aac (3)  II and aac (6 ‘)  Ib were 95%, 95%, 40% and 15% respectively]. Serial change appeared of amino acids in the sequence of armA of No.6, which identified itself as new subtype. Conclusion 95 % of the Acinetobacter baumannii isolates in the elderly patients