EGFR antisense oligonucleotide on human retinal glial cell migration.docVIP

 PAGE \* MERGEFORMAT 12 EGFR antisense oligonucleotide on human retinal glial cell migration [Abstract] Objective: To investigate the epidermal growth factor receptor (epidermal growth factor receptor, EGFR) antisense oligonucleotide (antisense oligonucleotide, ASODN) on human retinal glial (retinal glial, RG) cell migration. Methods: human retinal glial cells in primary culture, using liposome (Lipofectin) will be modified as a carrier-type EGFR ASODN RG transfected cells, using cell counting method for evaluating Murphy antisense oligonucleotide on human RPE cells in vitro migration . Results: liposome-mediated EGFR antisense oligonucleotides compared with the control group, cell migration activity was inhibited (P lt;0.05), 24h after transfection inhibition rate of 63.27%. Conclusion: EGFR ASODN can inhibit RG cell migration. [Keywords:] retinal glial cells; epidermal growth factor receptor; antisense oligonucleotide; liposomes; cell migration AbstractAIM: To observe the effect of antisense oligonucleotide (ASODN) hybridized epidermal growth factor receptor (EGFR) on migration of human retinal glial (RG) cells. ?r METHODS: Human RG cells were cultured in vitro. Modified EGFR ASODN was transfected into RG cells by lipofectin. By using an in vitro wound healing model in which a small area of #8203;#8203;a confluent monolayer of RPE cells was denuded, migration was measured by the number of cells that had entered the denuded area. ?r RESULTS: The cell migration was inhibited effectively by ASODN with lipofectin at 63.27%. The missense oligonucleotides showed no such effect (P lt;0.01). ?r CONCLUSION: EGFR ASODN can inhibit the migration of RG cells. KEYWORDS: retinal glial cell; epidermal growth factor receptor; antisense oligonucleotide; lipofectin; migration 0 Introduction Retinal glial (retinal glial, RG) cells in maintaining normal metabolism and function of the retina play an important role, but also participate in a variety of pathologic