Effect of simvastatin on H2O2-induced inhibition of cardiomyocyte apoptosis.docVIP

 PAGE \* MERGEFORMAT 13 Effect of simvastatin on H2O2-induced inhibition of cardiomyocyte apoptosis [Abstract] Objective of simvastatin on H2O2-induced myocardial cell apoptosis and its mechanism. Methods born 2 ~ 3 d of SD (Sprague-Dawley rats were cultured neonatal rat cardiomyocytes, with the establishment of myocardial H2O2 apoptosis model. randomly divided into five groups: normal group, injury group, 1 mol / L, 3 mol / L, 10 mol / L simvastatin protection group. injury group H2O20.2 mmol / L effect of 6 h. A based MTT assay (MTT method for the determination of myocardial viability, PI-AV staining, the flow cytometry apoptotic rate, measured by Western blot, caspase-3 protein content. Results (1) H2O2 can myocardial cell damage was caused: injury group compared with the normal decline in cell viability. simvastatin 1 mol / L, 3 mol / L, 10 mol / L protection group on the injured myocardial cells have a different degree of protection (P lt;0.05 ), and with the dose effect is obvious, (2, apoptosis rate by flow cytometry injury group than in the control group increased (P lt;0.05), simvastatin apoptosis rate of the protection group lower than the injury group (P lt; 0.05), and with the dose effect is obvious, (3 measured by Western blot, caspase-3 protein showed that injury group than in the normal group was significantly increased caspase-3, simvastatin can reduce the protection group H2O2 induced caspase-3 protein content increased, and with the dose effect is obvious. Conclusion Simvastatin can reduce the H2O2 induced apoptosis in cultured myocardial cells, inhibiting cell apoptosis-related protein caspase-3 related. [Keywords:] simvastatin myocardial cell apoptosis in caspase hydrogen peroxide Abstract: Objective To investigate the protective effect and mechanism of simvastatin on cultured cardiomyocytes apoptosis by H2O2. Methods About 2 ~ 3 days old Sprague-Dawley rat neonatal cardiac myocytes were cultured, the cultured cells were divided int