Effect of simvastatin on SH-SY5Y neural cells cholinergic nervous system stimulation.docVIP

 PAGE \* MERGEFORMAT 6 Effect of simvastatin on SH-SY5Y neural cells cholinergic nervous system stimulation [Abstract] Objective: To study the degradation of cholesterol drug simvastatin (Simivastatin) on SH-SY5Y neural cells, cholinergic nerve stimulation to explore the history of statin drugs in neurodegenerative diseases, neurological protection mechanisms. Methods: The cultured SH-SY5Y neural cells (derived from human neuroblastoma cells), in the culture medium by adding simvastatin cultured 48 h, then cells was determined by colorimetric MTT reduction rate and the cholinesterase activity, with Western blot was determined α 7 and α 3 nicotinic receptor protein levels, RT-PCR was determined receptor mRNA expression levels of the class. Results: Simvastatin can reduce the cultured cells, acetylcholinesterase, and butyrylcholinesterase activity (decreased 41.9% and 18.1%); enhance α 7 nicotine receptor subunit protein and mRNA expression levels (respectively 23% increase and 32%), but had no significant effect on the α 3. Conclusion: Simvastatin can stimulate the cholinergic nervous system, may have a neurological protective effects. [Keywords:] simvastatin; cholinesterase category; nicotine receptors; SH-SY5Y neuroblastoma cells, [Abstract] Objective: To investigate the effect of simivastatin on cholinergic nerve system and to understand neuroprotective mechanisms of the drug in neurodegenerative diseases. Methods: SH-SY5Y cells (human neuroblastoma cell line), were incubated with simivastatin for 48 hr. The MTT reduction and cholinesterase activity were measured by using photometric method; the expressions of nicotinic acetylcholine receptor (nAChR) subunits α 7 and α 3 at protein and mRNA levels were detected with Western blotting and RT-PCR techniques respectively. Results: Simivastatin inhibited the activities of acetylcholinesterase and butyrylcholinesterase by 41.9% and 18.1%, respectively, in the cultured cells. The drug increased the expressions of