Effect of sodium ferulate on high glucose-induced mesangial cell proliferation in primary impact.docVIP

 PAGE \* MERGEFORMAT 6 Effect of sodium ferulate on high glucose-induced mesangial cell proliferation in primary impact Author: SHI Ming-Juan Luo of Xiao Ying Guo Bing Zhang Guozhong [Abstract] Objective: To investigate the sodium ferulate (SF) induced by high glucose in primary glomerular mesangial cells (GMC) proliferation and its mechanism. Methods: Primary cultured glomerular mesangial cells were divided into normal group, mannitol group, high glucose group, high glucose plus different concentrations of SF groups, respectively, after treatment 24 h, 48 h cells were collected. With 4 - methyl thiazolyl tetrazolium (MTT) cell proliferation assay; immunocytochemical detection of p53 and p38 mitogen-activated protein kinase (p38MAPK) expression. Results: 48 h, the high glucose promotion of GMC proliferation and increase p38MAPK protein expression, the expression of p53 inhibition, SF significantly against the high-sugar this role, its role with the SF concentration increased, the role of the extension of time to strengthen. Conclusion: SF may be by inhibiting the p38MAPK pathway to promote the synthesis of p53, thus inhibiting the proliferation of high glucose-induced GMC. [Keywords:] diabetic nephropathy; protein p53; protein kinase; glomerular mesangial cells; sodium ferulate; p38 mitogen-activated protein kinase [Abstract] Objective: To investigated the effects and mechanism of sodium ferulate (SF) on the proliferation of mesangial cells induced by high glucose. Methods: The primary glomerular mesangial cells were divided into normal gloucose group, mannitol group, high gloucose group and high gloucose group plus different concentration of SF groups. Cells were collected at 24 h and 48 h of cultivation. Cell proliferation was measured by MTT assay. Expression of p53 and p38MAPK proteins was detected with immunocytochemistry method. Results: High glucose significantly promoted mesangial cell proliferation , up-regulated p38MAPK protein expression, and dow