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Effect of simvastatin on LPS-induced cell inhibition of mucus hypersecretion
PAGE \* MERGEFORMAT 13
Effect of simvastatin on LPS-induced cell inhibition of mucus hypersecretion
Of: Yang Jie Feng Yulin Gengyan Ming Qiao Yunfei
Abstract Objective To observe the anti-inflammatory activity of simvastatin with lipopolysaccharide (LPS)-induced A549 alveolar epithelial cell adhesion protein expression and its mechanism. Methods The effect of different concentrations of simvastatin in cultured A549 cells, using quantitative RT PCR, ELISA and gelatin zymography were used to detect cell adhesion protein expression levels of MUC5AC mRNA, MUC5AC protein content and cell supernatants of matrix metalloproteinase (MMP) 9 activity. Results in the simvastatin group cells and MUC5AC protein levels of MUC5AC mRNA were significantly decreased compared with LPS group (P lt;0.05), and the higher the concentration, the stronger the effect. Under normal circumstances, the supernatant of A549 cells undetectable MMP 9 activity, while the role of LPS in the supernatant after the MMP 9 activity was significantly enhanced (P lt;0.01), pretreatment with simvastatin were incubated with different concentrations of cell culture supernatant MMP 9 activity was significantly decreased (P lt;0.05). Conclusion LPS-induced A549 cell mucin MUC5AC mRNA and protein levels in the increase of MMP 9 activity and increased, whereas the simvastatin can reduce LPS-induced MUC5AC mRNA and protein expression in a dose dependent manner, suggesting that its role may be suppressed MMP 9 activity.
Keywords: simvastatin; lipopolysaccharide; sticky protein; alveolar epithelial cells
Mucus hypersecretion include chronic obstructive pulmonary disease (COPD), asthma, chronic airway inflammation, including an important pathophysiological features of disease. Because of excessive secretion of mucus and airflow obstruction may be aggravated as a medium for bacterial colonization and growth, resulting in decreased antibiotic effectiveness and becomes an independent influence morta
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