Effect of simvastatin on LPS-induced cell inhibition of mucus hypersecretion.docVIP

Effect of simvastatin on LPS-induced cell inhibition of mucus hypersecretion.doc

  1. 1、本文档共13页,可阅读全部内容。
  2. 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
  5. 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
  6. 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们
  7. 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
  8. 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Effect of simvastatin on LPS-induced cell inhibition of mucus hypersecretion

 PAGE \* MERGEFORMAT 13 Effect of simvastatin on LPS-induced cell inhibition of mucus hypersecretion Of: Yang Jie Feng Yulin Gengyan Ming Qiao Yunfei Abstract Objective To observe the anti-inflammatory activity of simvastatin with lipopolysaccharide (LPS)-induced A549 alveolar epithelial cell adhesion protein expression and its mechanism. Methods The effect of different concentrations of simvastatin in cultured A549 cells, using quantitative RT PCR, ELISA and gelatin zymography were used to detect cell adhesion protein expression levels of MUC5AC mRNA, MUC5AC protein content and cell supernatants of matrix metalloproteinase (MMP) 9 activity. Results in the simvastatin group cells and MUC5AC protein levels of MUC5AC mRNA were significantly decreased compared with LPS group (P lt;0.05), and the higher the concentration, the stronger the effect. Under normal circumstances, the supernatant of A549 cells undetectable MMP 9 activity, while the role of LPS in the supernatant after the MMP 9 activity was significantly enhanced (P lt;0.01), pretreatment with simvastatin were incubated with different concentrations of cell culture supernatant MMP 9 activity was significantly decreased (P lt;0.05). Conclusion LPS-induced A549 cell mucin MUC5AC mRNA and protein levels in the increase of MMP 9 activity and increased, whereas the simvastatin can reduce LPS-induced MUC5AC mRNA and protein expression in a dose dependent manner, suggesting that its role may be suppressed MMP 9 activity. Keywords: simvastatin; lipopolysaccharide; sticky protein; alveolar epithelial cells Mucus hypersecretion include chronic obstructive pulmonary disease (COPD), asthma, chronic airway inflammation, including an important pathophysiological features of disease. Because of excessive secretion of mucus and airflow obstruction may be aggravated as a medium for bacterial colonization and growth, resulting in decreased antibiotic effectiveness and becomes an independent influence morta

您可能关注的文档

文档评论(0)

jiupshaieuk12 + 关注
实名认证
文档贡献者

该用户很懒,什么也没介绍

版权声明书
用户编号:6212135231000003

1亿VIP精品文档

相关文档