EMB gene codon 330 and the fluorescent molecule detection of DNA probe design.docVIP

 PAGE \* MERGEFORMAT 12 EMB gene codon 330 and the fluorescent molecule detection of DNA probe design Of: Chen Qinghai, FU Wei Ling, Chun-Jiang Liu, Wei Wu, Hung Kuang [Abstract] Objective for Mycobacterium tuberculosis resistant to ethambutol embB330 codon DNA probe design elements, try to use direct observation of the liquid phase fluorescence spectrophotometer probe DNA molecules amplified products with embB330 codon fluorescence signal after hybridization to the detection of the mutation. Methods Beacon designer software design embB codon 330 gene contains DNA molecular probes, fluorescence spectrophotometer embB306 codon amplified fluorescence signal after hybridization with the probe, more amplification products were sequenced and the results. The results observed by fluorescence spectrophotometer and embB330 tuberculosis standard strain of mutant codons after hybridization with the probe PCR products were significant differences in the fluorescence signal, 33 ethambutol-resistant standards group and 10 strains of group H37RV Comparison of fluorescence signal strength, resistance to ethambutol group embB330 codon mutation detection rate was 3%, sequencing mutation detection rate of 3%. Conclusions of molecular DNA probe can detect single base embB330 target codon mutation , directly observed by fluorescence spectrophotometer liquid fluorescent hybridization signals simple and sensitive. [Keywords:] embB ethambutol-resistant gene, codon 330 mutation, molecular DNA probes, fluorescence spectrophotometer Abstract: Objective To design molecular DNA probe detecting embB330 codon of Ethambutol resistant mycobacterium tuberculosis (MTB), and to detect fluorescence of mutation site of embB330 codon in liquid by fluorescence spectrophotometer. Methods The software, beacon designer, was used to design molecular DNA probe detecting embB306 codon and detecting fluorescence signal from hybridization between the amplified product and probe by fluorescence s