Emei different altitude populations of quercetin content in Houttuynia.docVIP

 PAGE \* MERGEFORMAT 21 Emei different altitude populations of quercetin content in Houttuynia Author: Liu Lei, Wu Wei, Zheng Liang, Sandra Oh 【Abstract】 Objective To study the Emeishan populations at different altitudes Yuxingcao differences in quercetin content. Methods Hypersil ODS C18 (5 μm, 4.0 mm × 250 mm) column, mobile phase of acetonitrile: 0.4% phosphoric acid = 45:55, adjusted pH = 3, flow rate 1.0 ml / min, column temperature 30 ℃, UV detection wavelength of 370 nm. Results The method is simple, rapid, accurate, and can be used as Yuxingcao quantitative analysis of quercetin. Conclusion of 9 different elevation populations of quercetin Houttuynia there are significant differences in the levels and altitude and the correlation between soil nutrient content are not significant. Keywords: Houttuynia; quercetin; altitude; soil nutrients; RP-HPLC Abstract: ObjectiveTo study the difference of quercetin content of H.cordata Thunb. From different altitudes of Mt.Emei.MethodsQuercetin was determined by HPLC on Hypersil ODS C18 (4.0 mm × 250 mm, 5 μm) at 30 ℃ with acetonitrile: 0.4% phosphoric acid (45:55), pH = 3 as the mobile phase.Detection wavelengh was 370 nm.The flow rate was 1.0 ml / min.Results This method is simple, fast and accurate, and can be the quantitative analytic method of quercetin content of H. cndata.ConclusionThere are significant differences among the quercetin content of 9 populations from different altitudes.The correlationship between quercetin content and altitudes and soil nutrient are not significant. Keywords:: Houttuynia cordata Thunb.; Quercetin; Altitude; Soil nutrient; RP  HPLC Houttuynia is Saururaceae Houttuynia Houttuynia cordata Thunb. Of the whole plant. Widely distributed in central China, south-east and south-western provinces and autonomous regions, especially in Sichuan, Hubei, Hunan, Jiangsu and other provinces mostly. Of Weixin, cold, with heat detoxification, swelling