Eucommia Extract Experimental Study of Induced Mutation.docVIP

 PAGE \* MERGEFORMAT 4 Eucommia Extract Experimental Study of Induced Mutation Abstract] Objective To study the mutagenicity of Eucommia extract, provides a theoretical basis for their applications. Methods of mice fed with different doses of aqueous extract of Eucommia ulmoides, mouse bone marrow micronucleus test, sperm deformity rate and the use of Salmonella typhimurium reverse mutation test (Ames test) were observed. Results Eucommia extract solution right mouse bone marrow polychromatic erythrocytes micronucleus rate was not affected; on mouse sperm without deformity mutagenesis; right for the test strain no mutagenic effect. Conclusion Eucommia extract on subjects matter there was no significant mutagenic effect. [Keywords:] Eucommia micronucleus mutagenicity test Eucommia Eucommia ulmoides Oliv application in China has a long history in China in recent years, scholars in Eucommia biological, chemical composition, physiology, biochemistry, etc. Many studies have been done, DONG Juan-e et al [1] reported Eucommia seed oil has a blood pressure, blood cholesterol and anti-tumor and so on. Tang Jian-jun et al [2] reported: Eucommia is rich in vitamin E and β -carotene, have anti-aging, strengthen immunity effect. In order to better development and utilization of Eucommia, we explore the production Zhangjiajie Eucommia extract (referred to as Eucommia extract) the role of sub-chronic toxicology [3] on the basis of its mutagenic effect has been studied, designed for applications a certain theoretical basis. 1 Materials 1.1 Drugs with animals main component of Eucommia extract chlorogenic acid, Zhangjiajie Hengxing biotechnology companies. Kunming mice were purchased from Experimental Animal Center of Hunan Medical College, Batch number: 990,218. Injection with cyclophosphamide: Shanghai Hualian Pharmaceutical Co., Ltd., batch number: 030,504. Sodium azide (NaN3): Shanghai Reagent Plant, batch number: 990,706. Salmonella typhimurium strains (TA97, TA98, TA