Fatty degeneration of liver cells uncoupling protein 2 expression in.docVIP

 PAGE \* MERGEFORMAT 14 Fatty degeneration of liver cells uncoupling protein 2 expression in Of: Zhu Yanan, Feng Kun, Wang Bingfang, Ao Lin Ho [Abstract] Objective: To study the uncoupling protein 2 (UCP2 in normal liver cells and different degrees of fatty degeneration of liver cells. Methods: Different concentrations of fat emulsion using cultured hepatocytes, and made #8203;#8203;it different degrees of fatty change fatty degeneration of liver cells as a model. normal cells as the control group, with another three for the model group, the culture medium the concentration of fat emulsion were 0.5, 1.0,2.0 l / ml. application of RT PCR detection of UCP2 mRNA expression in cells the level of expression of UCP2 protein blot. Results: The normal liver cells can detect the expression of UCP2 mRNA, and with the increasing concentration of fat emulsion, fatty degeneration of liver cells relative expression of UCP2 mRNA was also increased. model group and normal expression of the control group significantly (P lt;0.01, between the expression of the model group were statistically significant differences (P lt;0.01. the normal group UCP2 protein was not detected, the model group with fatty degeneration of liver cells aggravation, UCP2 protein expression also increased (P lt;0.05. Conclusion: The fatty degeneration of liver cells, UCP2 expression increased compared with normal liver cells, and the expression with the severity of steatosis increases. [Keywords:] liver cells; expression; uncoupling protein 2; fatty degeneration [Abstract] Objective: To research the expression of uncoupling protein 2 in normal hepatocytes and fatty liver cells. Methods: Cultured hepatocytes with fatty emulsion, the cells got steatosis to varying degree and made them as the model of fatty liver cells. Normal cells used as control, cultured the model cells with medium containing different concentration of fatty emulsion as follows: 0.5,1.0,2.0 l / ml. Testified the UCP2 expressio