Felodipine on oxidative damage in human umbilical vein endothelial cells function.docVIP

 PAGE \* MERGEFORMAT 18 Felodipine on oxidative damage in human umbilical vein endothelial cells function Of: Qi Jie, Sheng Ying, Zheng Treasure, Zu-Yi Yuan [Abstract] Objective felodipine (felodipine) on oxidized low density lipoprotein (ox LDL) injury in human umbilical vein endothelial cells (HUVECs) reactive oxygen species (ROS) and expression of intercellular adhesion molecule 1 (ICAM 1), vascular cell adhesion molecule 1 (VCAM 1) mRNA expression, independent of blood pressure outside of its anti-atherosclerotic mechanism. HUVECs were screened ox LDL cells were incubated with optimal concentration gradient and time of intervention, and then with different concentrations of felodipine (0.1,1,10 mol / L) were incubated 24h, flow cytometry in ROS, real time PCR to detect cell ICAM 1, VCAM 1mRNA expression. Results ox LDL on ROS generation in HUVECs concentration dependent, with the stimulus concentration increased, ROS generation increased, 25mg / L ox LDL the most significant effect (P lt;0.01), felodipine inhibited ox LDL-induced HUVECs ROS (P lt;0.05) production; With ox LDL stimulated increase of the concentration, ICAM 1, VCAM 1 mRNA expression gradually increased, a concentration of 25mg / L, the role of the most significant (P lt;0.05), felodipine may be decreased ICAM 1, VCAM 1 mRNA expression (P lt;0.05). felodipine could inhibit the ox LDL-induced HUVECs ROS generation and reduced ICAM 1, VCAM 1 mRNA expression in endothelial play anti-inflammatory antioxidant protection. [Keywords:] felodipine; oxidized low density lipoprotein; oxidative stress; atherosclerosis; human umbilical vein endothelial cells; cell adhesion molecule 1; vascular cell adhesion molecule 1 ABSTRACT: Objective To investigate the protective effect of felodipine on reactive oxygen species (ROS) generation, mRNA level of inflammatory factors such as intercellular adhesion molecule 1 (ICAM 1) and vascular cell adhesion molecule 1 (VCAM 1), in human umbilical vein endothe