Forsythia orthogonal design of oleanolic acid extraction process.docVIP

 PAGE \* MERGEFORMAT 7 Forsythia orthogonal design of oleanolic acid extraction process [Abstract] Objective Optimization Forsythia oleanolic acid extraction process. Methods of oleanolic acid as an index, using L9 (34) orthogonal design experiments to study the extraction time, ethanol concentration, solid-liquid ratio on the extraction rate of the impact of the extraction process was optimized and reasonable. Determination of oleanolic acid by high performance liquid content. Results preferred the best out of oleanolic acid extraction conditions are: Ultrasonic extraction 3 times, 40 min / times, 90% ethanol solution, solid-liquid ratio 1:12. Conclusion This process extraction method is rapid, simple, and can provide a reference for further study of forsythia. [Keywords:] orthogonal oleanolic acid forsythia Abstract: ObjectiveTo optimize the extraction condition of oleanolic acid from Fructus Forsythiae. MethodsWith oleanolic acid as index, extraction time, concentration of alcohol and amount of alcohol and were evaluated by L9 (34) orthogonal design method. HPLC was used to analyze the content of oleanolic acid. ResultsThe optimum extraction condition was described as follow: ultrasonic extraction 3 times (once 40 min), 90% ethanol solution, and 12-fold solvent. ConclusionThe optimized technology is quick and simple, and can be used for theoretical basic. Keywords:: Orthogonal design; Oleanolic acid; Fructus Forsythiae Chinese medicine is the Oleaceae Forsythia Forsythia species of dry fruit, the main producing Shanxi, Henan, Shaanxi, there are broad-spectrum antibacterial effect, such as Staphylococcus aureus, hemolytic streptococcus, Streptococcus pneumoniae and Pseudomonas aeruginosa have different degrees of inhibition, there is antiemetic, antipyretic, anti-inflammatory and hepatoprotective effects, contains phillyrin, arctiin, oleanolic acid, etc. [1]. Oleanolic acid has a lower alanine aminotransferase and aspartate aminotransferase activity, p