Fourier transform ion cyclotron resonance mass spectrometry analysis and identification of peptide fragments of osteopontin.docVIP

 PAGE \* MERGEFORMAT 15 Fourier transform ion cyclotron resonance mass spectrometry analysis and identification of peptide fragments of osteopontin Of: Jiang Xinping Liu Cheng Ge in Leiwang Shao Mei Shilei [Abstract] using high performance liquid chromatography (HPLC) SCX and C4 column samples from human milk whey part of the isolated and purified OPN (Osteopontin, OPN), under certain conditions, be digested with trypsin, Using high-resolution Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS), combined with micro-spray (Nano spray) technology and database with Mascot software, access to the OPN-related peptide fragments GDSVVYGLR and QNLLAPQTLPSK the mass structure of information, analysis of the fragmentation mechanism . studies show that use of FT ICR MS combined with HPLC and other chemical methods, can be highly efficient and rapid separation and purification of human milk samples of OPN, a database with Mascot MS software can clean and accurate identification of peptide fragments related to the structural information for OPN peptide fragments of different post-translational modification sites to determine the relationship between biological activity and its study provides a theoretical basis. [Keywords:] Fourier transform ion cyclotron resonance mass spectrometry, human milk, osteopontin, peptide fragments 1 Introduction With the advances in modern biotechnology and life science development, peptide in vivo physiological function of more and more attention, the product obtained from natural bioactive peptide substances with the method has evolved. OPN (Osteopontin, OPN ) is an important multifunctional extracellular matrix protein [1], in bone cells, macrophages, epithelial cells express a variety of cells [2], in mediating cell chemotaxis, adhesion, proliferation and migration, and bone mineralization and reconstruction, immune regulation, signal transduction, plays an important role [3,4]. OPN is an arginine glycine w