Gang Song in the Determination of myricetin.docVIP

 PAGE \* MERGEFORMAT 8 Gang Song in the Determination of myricetin Of: Chen Mingsheng, Bu-Ming Liu, Lin Xiao, Cai Quanling [Abstract] Objective Gangsong are analyzed, measured in Myr Gangsong content quality standards for the gang loose to provide method and basis for the development. Methods using high performance liquid chromatography (HPLC) Determination of myricetin content Gangsong column was Diamonsil C18 (4.6 mm × 250 mm, 5 μm); mobile phase of acetonitrile - methanol - water - phosphoric acid (17.5:17.5:65:1.3); detection wavelength 360 nm; column temperature was room temperature; flow rate of 1 ml / min. Results bayberry factors in the range of 0.204 ~ 1.224 g was linear (r = 0.999 7), the average recovery was 97.12%, RSD was 1.50% (n = 6). Conclusion The method is simple and reproducible and can be used post Song Radix method for determination of myricetin. [Keywords:] Gang Song; myricetin; high performance liquid chromatography Abstract: ObjectiveTo develop a method for determination of myricetin content in natural herb of Baeckea frutescens L. and provide a reliable technique for the establishment of the quality standard of Baeckea frutescens L.. MethodsAn HPLC method was set up to determine the content of myricetin in Baeckea frutescens L. Diamonsil C18 (4.6 mm × 250 mm, 5 μm) column was adopted as the solid phase, and acetonitrile-methanol-water-phosphoric acid (17.5:17.5:65:1.3) was used as the mobile phase, the UV detection wavelength was 360 nm with a flow rate of 1.0ml/min at room temperature. ResultsA good linear relationship was obtained within the range of 0.204 ~ 1.224 g and the average recovery was 97.12%, RSD = 1.50% (n = 6) . ConclusionThe method is simple, reproducible and can be used for the determination of myricetin in Baeckea frutescens L. Keywords:: Baeckea frutescens L.; Myricetin; HPLC Gang Myrtaceae pine Pinus Kong Kong song Baeckea frutescens L., mainly distributed in Guangxi, Guangdong, Jiangxi, Fujian and o