Ginaton pairs of in vitro hypoxia-induced human aortic endothelial cells to secrete endothelin.docVIP

 PAGE \* MERGEFORMAT 7 Ginaton pairs of in vitro hypoxia-induced human aortic endothelial cells to secrete endothelin Authors: gao, MA Gui-Xi, Han Lei, Liu, Li Jing, Meng Guo, Liu Xin [Keywords:]; Endothelins class Effects of Ginaton on hypoxiainduced endothelin secretion from human aortic endothelial cells [Abstract] AIM: To explore the effects of Ginaton at different concentrations on the hypoxiainduced endothelin (ET) secretion from cultured vascular endothelial cells (VECs). METHODS: Cultured VECs were divided into 5 groups: control group, simple hypoxia group, highconcentration Ginaton group, moderateconcentration Ginaton group, and lowconcentration Ginaton group. For hypoxia, all groups except control group were put in a hypobaric chamber at the height of 5000 m for 30 min. The ET contents in supernatant of VECs before hypoxia and 0.5 h after hypoxia, 6 h after hypoxia as well as 24 h after hypoxia were measured with radioimmunoassay. RESULTS: ① Simple hypoxia promoted VECs secretion of ET and ET content increased significantly at 0.5 h after hypoxia and reached the peak at 6 h (P amp;lt;0.01). ② Ginaton inhibited VECs secretion of ET induced by hypoxia. At the time of 0.5 h after hypoxia, highconcentration Ginaton was more effective than moderateconcentration and lowconcentration Ginaton (P amp;lt;0.01). At the time of 6 h and 24 h after hypoxia, moderateconcentration Ginaton was more significantly effective than highconcentration and lowconcentration Ginaton (P amp;lt;0.05). CONCLUSION: Ginaton has significant protective effects on hypoxiainduced ET secretion from cultured VECs and the effects of moderateconcentration Ginaton are more obvious compared with those of highconcentration and lowconcentration Ginaton. [Keywords] endothelial, vascular / cytology; endothelins; ginaton; anoxia [Abstract] Objective: To study the effect of Ginaton hypoxia induced by the cultured vascular endothelial cells to secrete endothelin. Methods: Cultured vascular e