Glucosamine sulfate on cartilage cell proliferation in rats.docVIP

 PAGE \* MERGEFORMAT 14 Glucosamine sulfate on cartilage cell proliferation in rats [Abstract] Objective: To investigate the glucosamine sulfate (GS) on the proliferation of cartilage cells in rats. METHODS: The rat secondary enzyme digestion of articular cartilage cells were subcultured, and the fourth generation of cells for drug intervention by MTT colorimetric, immunohistochemical staining of proliferating cell nuclear antigen (PCNA, cell cycle detected by flow cytometry and other methods to detect the generation of times rat chondrocytes and drug intervention of the fourth generation of chondrocyte proliferation. Results: The rat cartilage With the passage culture cells, MTT colorimetry showed decreased proliferation and immunocytochemical detection of PCNA expression is reduced, flow cytometry showed cell proliferation index decreased, glucosamine sulfate can inhibit this trend and promote proliferation of subcultured capacity. Conclusion : GS on mass cultured rat chondrocytes obviously promote proliferation. [Keywords:] glucosamine sulfate, rat, cartilage cells, proliferation Abstract Objective: The purpose of the present paper was to investigate the effect of glucosamin sulphat (GS) on the proliferation of rat chondrocyte in vitro. Methods: The rat articular chondrocytes were isolated by enzyme digestion and sub-cultivated in serial. The 4th chondrocyte was interfered with GS. The chondrocytes of different generations and the 4th medicine intefered generation were detected with such methodsas PCNA (proliferating cell nuclear antigen), MTT (methyl thiazolyl tetrazolium) assay for proliferation and Flow cytometry for analyses of cell cycle distribution and PI ( proliferation index). Results: With rat chondrocytes sub-cultivated, MTT assay showed that the proliferating ability of chondrocytes decreased, the express of PCNA tapered in the experiment of immunohistochemistry, as well as PI descended in flow cytometry for analyses. GS can inhibit the