Gluteal muscle contracture in the pathogenesis of TGF1 and HSP47 expression and role of.docVIP

 PAGE \* MERGEFORMAT 13 Gluteal muscle contracture in the pathogenesis of TGF1 and HSP47 expression and role of Of: Zhao Chenguang, He Xijing, Lu Bin, Li Haopeng [Abstract] Objective To explore the transforming growth factor 1 (transforming growth factors 1, TGF 1) and heat shock protein 47 (heat shock protein, HSP47) in the GMC with its expression in the gluteal muscle contracture (gluteal muscle contracture , GMC) the role of disease. Methods of gluteal muscle contracture and lesions in patients with contracture of the muscles next to the pathological specimens, using reverse transcription - polymerase chain reaction (RT PCR), immunohistochemistry and Western blot detection of TGF 1 and HSP47 in mRNA expression and protein levels. Results TGF 1 and HSP47 localized in fibroblasts and the cytoplasm of endothelial cells, and showed strong positive expression, and its mRNA levels were increased by 8.1 and 3.6 times (P lt;0.05 .) protein and mRNA expression levels of the same, respectively, 11.2 times and 7.6 times increased (P lt;0.05). Conclusions The expression of TGF 1 and HSP47 may be caused by increased production of the important mechanisms with contracture. [Keywords:] gluteal muscle contracture, fibrosis, TGF 1, HSP47 ABSTRACT: Objective To explore the expression and effect of transforming growth factors 1 (TGF 1) and heat shock protein (HSP47) on gluteal muscle contracture (GMC). Methods We collected contraction band and adjacent muscle from GMC patients and explored the expression of TGF 1 and HSP47 using immunohistohemistry, reverse transcription and polymerase chain reaction (RT PCR), and Western blot analysis. Results TGF 1 and HSP47 were intensely expressed in fibroblast cells and vascular endothelial cells. The expression of them increased 8.1 fold and 3.6 fold at the mRNA level, respectively (P lt;0.05). The same changes were found at the protein level, which increased 11.2 fold and 7.6 fold, respectively (P lt;0.05). Conclusion Up reg