Goose grass roots determination of major saponins.docVIP

 PAGE \* MERGEFORMAT 9 Goose grass roots determination of major saponins Of: Hanlin Tao, Huang Fang, Zhou Yunyun, I still work, Fang Nianbo [Abstract] Objective To analyze the determination of saponins from Schefflera one of the major grass anhuienoside E content. Methods C18 column (Waters Corporation, Xterra RP18 3.5 m, 4.6 mm 150 mm), mobile phase was methanol - water (58 : 42), flow rate 1 ml / min, detection wavelength 209 nm. Results saponin anhuienoside E in the range of 2.14 ~ 10.70 g sample volume and the peak area was a good linear relationship (r = 0.999 6), the average recovery was 100.6 %, RSD = 1.28% (n = 6). Conclusion The method is simple, sensitive, reproducible and can be used as the main saponins tulip grass anhuienoside E method for the determination. [Keywords:] Anhuienoside E, Goose grass, high performance liquid chromatography Abstract: ObjectiveTo determine anhuienoside E in Rhizome of Anemone flaccida by HPLC. MethodsAnhuienoside E was separated on Xterra RP18 3.5m column (4.6 mm 150 mm) with a mobile phase of methanol-water (58:42) and detected at a wavelength of 209 nm. ResultsThe calibration curve was linear in the range of 2.14 ~ 10.70g (r = 0.999 6). The average recovery rate was 100.6% and RSD = 1.28% (n = 6). ConclusionThe method is simple, sensitive and precise. It can be used for the determination of anhuienoside E in Rhizome of Anemone flaccida. Keywords:: Anhuienoside E, Anemone flaccida, HPLC Goose grass Anemone flaccida Fr. Schmidt, Department of perennial Ranunculaceae Anemone. The genus of about 150 species, are distributed on every continent, China has 54 species, with the exception of Hainan province, other provinces are distributed [1, 2], where tulip grass widely distributed in Hubei Province [1]. This can be used for medicinal plants have bamboo Cyperus A. raddeana, tulip grass A. flaccida, Anemone rivularis A. rivularis, etc. several, mostly root or rhizome medicine, with rheumatism, swelling