Gui Zhi Tang on Spleen Deficiency Rats NFAT mRNA IL4 mRNA IFNγ mRNA intervention effect.docVIP

 PAGE \* MERGEFORMAT 7 Gui Zhi Tang on Spleen Deficiency Rats NFAT mRNA IL4 mRNA IFNγ mRNA intervention effect [Abstract] Objective: RT  PCR method to explore the Spleen Gui Zhi Tang on NFAT mRNA, IL  4 mRNA, IFN  γ mRNA, as well as the impact of intervention in Th1/Th2 cell drift mechanism. Methods: 40 rats were randomly divided into two blank control group (A group), spleen group (B group), Gui Zhi Tang high-dose group (C group) and Gui Zhi Tang low-dose group (D group) and other four groups, each 10; with the norms of spleen-deficiency Permit rat model modeling method B, C and D rats modeling, B, C and D respectively with distilled water, Gui Zhi Tang high-dose, low-dose gavage Gui Zhi Tang, specimen collection later RT  PCR Detection of NFAT mRNA, IL  4 mRNA and IFN  γ mRNA expression. Results: Spleen group of NFAT mRNA, IL  4 mRNA upregulation; and IFN  γ mRNA expression reduced by Gui Zhi Tang large, low-dose group after treatment , NFAT mRNA, IL  4 mRNA expression in down, IFN  γ mRNA upregulation. Conclusion: Gui Zhi decoction on the role of Th1/Th2 cells, either through the drift down the expression of NFAT mRNA restored normal IFN  γ mRNA transcription levels, further reduced IL  4 mRNA expression, so that Th1/Th2 cells in rat spleen to reach a new equilibrium. [Keywords:] Gui Zhi Tang; activated T cell nuclear factor; IL  4; γ interferon 0 Introduction Modern medicine shows that under physiological conditions, Th1 cells and Th2 cells to secrete the two types of factors to adjust with each other, mutual restraint, in a dynamic equilibrium state. Once the imbalance between the two cytokines act on the cell surface corresponding receptors, the activation signal transduction factor (signal transducer factor, STF), will stimulate the signal transmitted to the nucleus and play its role in the regulation of gene transcription, so that the body of local and even systemic pathological response [1]. we have observed spleen