Han vitamin D receptor gene Tru and Fok polymorphism and the relationship between lumbar disc degeneration.docVIP

 PAGE \* MERGEFORMAT 27 Han vitamin D receptor gene Tru and Fok polymorphism and the relationship between lumbar disc degeneration Author: Chen Jian Yeh Wei-Yue Ding Supei Jiang Li Gui-tao HUANG Dong-sheng LIU Shang Abstract [Objective] To investigate the Han people of vitamin D receptor (vitamin D receptor, VDR) gene polymorphism and lumbar disc degeneration (lumbar disc degeneration, LDD) relationship. [Method] collected 182 cases of Han Chinese blood specimens and lumbar MRI, of which 101 cases in control group, case group of 81 cases. Polymerase chain reaction - restriction fragment length polymorphism (PCR  restriction fragment length polymorphism, PCR  RFLP) Determination of Group 2 specimens Tru Ⅰ and the VDR gene Fok Ⅰ restriction site polymorphism; according to MRI show differences in the signal by Schneiderman classification method to determine the individual lumbar intervertebral disc degeneration, sub-free, light, medium and heavy 4 groups. Analysis of case-control group, genotype, allele frequency distribution, analysis of which less than 45 years of age (including 45 years of age) genotype and gene frequency distribution and the relationship between the degree of disc degeneration. [Results] The control group Fok Ⅰ and Tru Ⅰ allele frequency distribution of: F 59.4%, f 40.6% and T 79.2%, t 20.8%; case group Fok Ⅰ and Tru Ⅰ allele frequency distribution of: F 53.7% , f 46.3% and T 80.9%, t 19.1%, the second group, no significant differences in the distribution, Pgt; 0.05; in the MRI group in the VDR gene Tru Ⅰ and Fok Ⅰ restriction site of the genotype and allele frequencies of The distribution in the group and no significant difference, Pgt; 0.05. [Conclusion] VDR gene Tru Ⅰ and Fok Ⅰ restriction site polymorphism and LDD Han nationality has nothing to do. Keywords: vitamin D receptor gene single nucleotide polymorphism restriction fragment length polymorphism of lumbar disc degeneration Association of vitamin D receptor