Hedyotis diffusa ethanol extract of the lung adenocarcinoma A549 cell line proliferation and apoptosis.docVIP

 PAGE \* MERGEFORMAT 8 Hedyotis diffusa ethanol extract of the lung adenocarcinoma A549 cell line proliferation and apoptosis Author: Gao Bao, Chen Shixiong, Yang Jun, Huang Ji, Hong-Yan Deng [Abstract] Objective To study the ethanol extract of Oldenlandia diffusa in vitro of lung adenocarcinoma A549 cell line proliferation and apoptosis and its mechanism. Methods MTT determination of different concentrations of ethanol extract of Oldenlandia diffusa on A549 cell proliferation; Hoechst 33258 detection After the effects of ethanol extract of Oldenlandia diffusa apoptosis; flow cytometry cell cycle after the drug action and apoptosis; Western Blot Detection of drug effect and Bax after the Bcl-2 protein expression. The results of ethanol extract of Oldenlandia diffusa inhibit A549 cell proliferation in the amount of time   efficiency and effect relationship; Hoechst 33258 detection of ethanol extract of Oldenlandia diffusa role of apoptotic cells in A549 cells; 40 mg / ml ethanol Hedyotis diffusa Extract the role of A549 cells 12,24,36,48 h, drug group the percentage of G1 ~ G0 phase cells, respectively (42.82 ± 3.95)%, (51.84 ± 6.37)%, (54.76 ± 8.85)% and (64.94 ± 7.56)% Drug cell apoptosis rates were (5.72 ± 0.98)%, (7.10 ± 2.08)%, (19.89 ± 4.25)%, (32.55 ± 6.51)%, were higher (P all amp;quot;0.05), and G1 ~ G0 phase cells and apoptosis rate with the percentage of time the increase of drug effects; Western Blot showed that ethanol extract of Hedyotis diffusa role in A549 cells, Bax expression increases with the role of the time, Bcl-2 expression with the role of the time to reduce the increase. Conclusion ethanol extract of Hedyotis diffusa and the role of drug concentration with time, to increase lung adenocarcinoma A549 cells in vitro inhibitory effect of an increase; its inhibition may be through cell cycle arrest in G1 ~ G0 phase and by up-regulating Bax and down-regulation Bcl  2 expression and the induction of apoptosis to achieve. [Keywo