Hemolytic lecithin on endothelial cells in the regulation of CD73.docVIP

 PAGE \* MERGEFORMAT 2 Hemolytic lecithin on endothelial cells in the regulation of CD73 [Keywords:] endothelial cells, extracellular adenosine 5   Nase 1 Materials and methods 1.1 Materials Cell culture dishes, culture medium and the cell columns were purchased from Promocell companies; tests reagents vinyl adenosine monophosphate (eAMP), lysophosphatidylcholine (LPC), celandine red base, AOPCP (α  β   methyl-adenosine 5   diphosphate), NBTI (n   nitrobenzyl thio-hypoxanthine nucleotides) were purchased from Sigma Corporation. 1.2 Method Method Isolated and cultured PAEC, derived from the third generation of PAEC used in the experiment. Use of Deussen et al [1] first described the method of the cell column, analysis of adenosine secretion measured PAEC. This paper carried out two experiments, each using 10 cell column. Every 3 min continue to collect an outflow of fluid, each observation of the object collection of five out of fluid. a group of cell columns successively pumped into eAMP (5 μ mol / L), adenosine membrane transport inhibitor (NBTI, 1 μ mol / L), LPC ( 25 μ mol / L) and extracellular 5   foreign nucleotide inhibitors (AOPCP, 50 μ mol / L). Another group of cell columns sequentially pumped into the following reagents: eAMP (5 μ mol / L), NBTI (1 μ mol / L), protein kinase C (PKC) inhibitor, celandine red base (100 μ mol / L), LPC (25 μ mol / L). application of high-performance liquid chromatography (HPLC) determination of adenosine and ethylene  gland glycoside content. Extraction of cell columns in all of the PAEC, in order to Lowry et al [2] determined by the method described in its protein content. 2 Results Two groups of cell columns in only when the pump eAMP cell column in the PAEC endogenous synthesis of adenosine (5.0 ± 1.8) pmol / min. mg-1. NBTI to make this value significantly increased to (9.7 ± 3.9) pmol / min * mg-1 (P