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Heparin anticoagulation on plasma biochemical test results of the 10 comparison
PAGE \* MERGEFORMAT 5
Heparin anticoagulation on plasma biochemical test results of the 10 comparison
Keywords:] heparin plasma are the detection of biochemical
In clinical biochemistry, our practice of using a variety of serum biochemical testing, but the serum time-consuming and laborious preparation, so in recent years, many foreign experts on the detection of different anticoagulants in plasma biochemical parameters studied in detail. I anticoagulation with heparin plasma were 10 biochemical test, the results reported below.
1 Materials and Methods
1.1 The selection of blood specimen collection
49 patients to the hospital where the medical writer who, according to conventional methods venous blood 4ml, drying tube into the common 2ml, after 30min at 37 ℃ water bath, centrifuge the serum, the other tube 2ml injection of heparin anticoagulation (heparin sodium content of blood To 25U/ml), plasma centrifuge immediately after mixing.
1.2 Analysis
After the centrifugal separation of plasma and serum immediately GLU, CHOL, TG, Cre, BUN, UA, ALT, AST, CK, TBIL total of 10 biochemical detection. Use of instruments for the Roche Modular P automatic biochemical analyzer, reagents provided by the Roche company, and by Roche cfs and Precinorn u and Precipath u calibration and quality control material for internal quality control.
1.3 Data Processing
SPSS10.0 software used for statistical, measurement data were analyzed by paired t test and χ2 test data.
2 Results
Serum and heparin plasma are the result of 10 biochemical parameters in Table 1. Table 1 of the 10 serum and plasma biochemical results can be seen from Table 1, plasma glucose and serum total bilirubin test results than the significantly increased (P lt;0.01), while the remaining eight plasma and serum biochemical test results no significant difference (Pgt; 0.05). Correlation analysis showed that plasma and serum results are highly correlated.
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