Hepatitis B virus large surface protein on its clinical significance.docVIP

 PAGE \* MERGEFORMAT 7 Hepatitis B virus large surface protein on its clinical significance Hepatitis B virus (HBV, hepatitis B virus large surface protein present in infectious particles (Dane particles) and sub-viral particles tube, is a virus an important indicator of a complete membrane, and viral replication is closely related with the trans-activation, and with the release of virus particles from cells related to [1]. Some studies show that the HBV large protein has a dual transmembrane topology [2], for the HBV large protein conformation specific monoclonal antibody preparation, a more accurate detection of serum hepatitis B virus large protein. In order to understand hepatitis B patients with serum HBV DNA HBV large protein correlation of 179 cases of HBV infection in our serum HBV large protein were detected. 1 Materials and methods 1.1 Case study of 179 patients for the hospital in June 2008 -2009 hospitalized patients in November, male 122 cases, 57 cases of women, aged 17-65 years, mean age 36 + -12.5 years old, meet the diagnostic criteria of the Chinese in 2000 Medical units will be infectious and parasitic diseases, liver credits will be jointly revised “viral hepatitis prevention and treatment programs” in the diagnostic criteria for viral hepatitis. 1.2 specimens from blood samples collected by the vacuum blood serum was separated after the stored frozen at -20 ℃. 1.3 Reagents HBV DNA was detected by Zhongshan University, Guangzhou Da An Gene Co., Ltd. reagents, HBV “two pairs of half” were detected by time-resolved fluorescence immunoassay, reagents purchased Biotechnology Co., Ltd. Suzhou New Wave, HBV large protein detection reagents from Beijing Thermal King Biotech Limited. The principal means of HBV-LP 1.4 reagent application of structural biology and immunology techniques, screening for hepatitis B virus large surface protein three-dimensional conformation of the former S-type area of #8203;#8203;high epit