High performance liquid chromatography - electrospray ion trap time of flight mass spectrometry identification of metabolites Mequindox.docVIP

 PAGE \* MERGEFORMAT 19 High performance liquid chromatography - electrospray ion trap time of flight mass spectrometry identification of metabolites Mequindox Study: Red Bin He Limin Liu Yingchun Division in Ding Huan Huang Chen was the rod will be the new pomegranate ZENG Zhen Chen Ling [Abstract] using high performance liquid chromatography, electrospray ionization - linear ion trap mass spectrometry (LC-ESI/LTQ, electrospray ionization - ion trap / time of flight tandem mass spectrometry (LC-ESI/IT-TOF identification of chicken plasma Mequindox in the structure and its metabolites. respectively, and Symmetry ShieldTM Hypersil Gold column, containing 0.01% formic acid (A and methanol (B as the mobile phase, gradient elution, electrospray ionization (ESI positive ion patterns were detected. Mequindox chicken oral administration (20 mg / kg, the wing vein blood until the analysis of plasma with acetonitrile - ethyl acetate (3:2, V / V mixture extracted dry. residue 30% methanol solution reconstituted, the machine testing. with Shimadzu’s Composition Molecular prediction software suggested the molecular ion and product ion mass, Xcalibur 2.0.7 software to analyze linear ion trap mass spectrometer measured mass spectrum. identified the main metabolite of 5 , respectively, 3 - methyl -2-- (1 - hydroxyethyl - quinoline -1, N4-dioxide (M1, 3 - methyl -2-- (1 - hydroxyethyl - quinoline-N4-A oxide (M2, 3 - methyl -2-- acetyl - a quinoline-N4-oxide (M3, 3 - methyl -2-- acetyl - quinoline (M4 and 3 - hydroxymethyl -2-- ( 1 - hydroxyethyl - quinoline-N1, N4-dioxide (M5. speculated that the results under both metabolites M1 ~ M4 for chemical synthesis, synthetic metabolites measured spectral data further confirmed the chemical structure of metabolites suggested . this experiment to study the metabolism Mequindox and metabolic mechanisms of the body provides a convenient and effective model. [Keywords:] Mequindox; metabolites; high performance liquid chromatograp