High Performance Liquid Chromatography 5 plus Velvet Oral Liquid in the content of isofraxidin.docVIP

 PAGE \* MERGEFORMAT 9 High Performance Liquid Chromatography 5 plus Velvet Oral Liquid in the content of isofraxidin [Abstract] Objective: To determine the five plus Velvet Oral Liquid in the different levels of pyridine hydrochloride. Methods: The high-performance liquid chromatography, in order to ODSC18 column (150mm * 4.6mmID, 5μ m) as the stationary phase, acetonitrile -0.1% phosphoric acid (20:80) as mobile phase, detection wavelength of 342nm. Results: iso-triazine pyridine in 18.752 ~ 187.52 * 10 -3 μ g showed good linearity within the framework of the correlation coefficient r = 0.9997. The average recovery of 95.26%, RSD of 1.31% (n = 6). Conclusion: The method is simple, accurate and reproducible. [Keywords:] isofraxidin; high-performance liquid chromatography; Ciwujia 5 plus Velvet Oral Liquid prescription from Acanthopanax, deer horn and blood, velvet, vitamin B 1 formed with several accessories. With the brain by the marrow, blood Zhuang waist, sleep strong stomach, kidney to keep fit, refreshing rejuvenation achievements. For the physically weak anemia, impotence and kidney cold, neurasthenia. Fang acanthopanax contains a number of saponins, alkaloids, one of the main component isofraxidin, and its ingredients are relatively easy determination of the content. In this paper, five Canadian Velvet main active ingredient of blood isofraxidin as an index, using high performance liquid chromatography with acetonitrile, phosphoric acid as mobile phase, determination of its content, the method is simple, accurate, high precision, reproducibility Well, for the control of quality standards provide a reliable basis. An instrument and reagent Shimadzu LC-10Avp series of high-performance liquid chromatograph, SPD-10Avp UV detector; CLASS-VP data processor; reference substance isofraxidin (Lot No. :0837-200203; for the assay used by the Chinese Pharmaceutical and Biological Products offered by test). Methanol, acetonitrile HPLC pure water as water fo