High Performance Liquid Chromatography Antongding vine in the content of lupeol.docVIP

 PAGE \* MERGEFORMAT 10 High Performance Liquid Chromatography Antongding vine in the content of lupeol Author: DENG Peng Xie Yihui Zhang Ye Yu Musou [Abstract] Objective To establish Antongding Fujinaka lupeol content determination. Methods using high performance liquid chromatography (HPLC) method, column for the diamonsil C18 (5 μ m, 250 mm * 4.6 mm), mobile phase was methanol: water = 98:2, flow rate 1.0 ml * min-1, detection wavelength 206 nm The column temperature was 30 ℃ . The results of lupeol in the 0.316 ~ 3.16 μ g and the peak area within the framework of a good linear relationship, r = 0.999 5, the average recovery was 97.82%, RSD 2.49%. Conclusion This method of determination of Antongding vine lupeol content fast, simple, accurate and reliable. [Keywords:] high-performance liquid chromatography Antongding vine lupeol On pain vine for Vitaceae Cissus Long vine plant suffered Cissus assamica (Laws.) of the vine stem, with Qufengchushi, Sanyu, Sida role, attending rheumatism Bitong, bone-sprains, swollen ulcer drugs, Jiangxi and other places of habitual use medicinal treatment of rheumatoid arthritis. It is mainly distributed in South China, Southwest China and Jiangxi, Fujian, Tibet and other places [1]. Pharmacological experiments show that Antongding vine has good anti-rheumatoid effects [2]. I was isolated from an identification of pain and rattan lupeol and other ingredients. It is reported in the literature, lupeol in experimental animals have anti-inflammatory [3], anti-oxidation [4], promote skin healing [5] and other pharmacological effect, it is a preliminary to determine lupeol is an effective component of pain, one of the vine ( Pharmacological experiments in progress). To this end, it is proposed lupeol vine in pain as an indicator of quality control. At present, lupeol assay methods include TLC [6], high-performance liquid mass spectrometry [7], but also no HPLC method of determination of lupeol content of reports. In order to qua