Hepatitis B virus associated with primary liver cancer in patients with preX gene in molecular epidemiology of meaning.docVIP

 PAGE \* MERGEFORMAT 2 Hepatitis B virus associated with primary liver cancer in patients with preX gene in molecular epidemiology of meaning [Abstract] Objective: To understand the pre  X gene in HBV-related HCC patients in the epidemiological distribution, before  X gene and HBV genotype and the clinical relationship between HCC. Methods: suffering from hepatitis B patients with hepatocellular carcinoma (HCC) 35 cases, using type-specific primers by nested polymerase chain reaction genotyping. the same time, a clear genotyping of liver cancer patients, amplified by the former  X Ou, TA cloned into the vector after PMD18  T monoclonal sequencing, Analysis of pre  X zones in the expression of liver cancer patients, the former  X area and the genotype and related illness research. Results: HCC patients with 35 patients with genotype B in 1 case, C genotype of 24 patients, B / C Mixed genotype in 8 cases, undifferentiated type 2 cases. 33 cases of patients with specific genotypes, and further expansion of the former  X zone, and after TA cloned 93 monoclonal lines by sequencing showed that there are 65 strains encoded monoclonal pre  X peptides, accounting for 69.9%. HCC 33 patients there were 29 cases before coding  X polypeptide, encoding rate of 87.9%, higher than the 58.8% in patients with chronic hepatitis B encoding rate. C genotype before  X polypeptide encoded rate was 91.6%, B / C hybrid coding rate of 87.5% (P