Hemangioblastoma of the central nervous system cells and between neurons in cell protein analysis.docVIP

 PAGE \* MERGEFORMAT 21 Hemangioblastoma of the central nervous system cells and between neurons in cell protein analysis Study: Hang Xie Rong Liu Jun Yao Zhu Jianhong Yang Peng-Yuan Fan Huizhi [Abstract] the artificial cultivation of the central nervous system hemangioblastomas (Central nervous system hemangioblastoma, HB) cells to study the development of the proteomics analysis, identified the HB cells and neurons in different cell proteins. By On-line HPLC LTQ Orbitrap tandem mass spectrometry identification of soluble protein samples obtained HB cell proteome expression profiling. HB 674 cells were identified by the protein, neurons obtained 531 proteins identified. According to peptides identified based on semi-quantitative analysis of proteome Methods of mass spectrometry data for comparative analysis of different proteins, found in vimentin (Vimentin), 14 3 3 epsilon protein and carbonic anhydrase Ⅱ (Carbonic anhydrase Ⅱ, CA Ⅱ) and other cells in the HB significant change in the expression of the protein, and characterized by immunohistochemical staining. The results show that vimentin (Vimentin), 14 3 3 epsilon protein and carbonic anhydrase Ⅱ (CA Ⅱ) and other protein expression changes and the incidence of HB is closely related to the exploration of HB the origin is important. [Keywords:] the central nervous system hemangioblastomas, proteomics, high performance liquid chromatography mass spectrometry, semi-quantitative analysis 1 Introduction Changes in protein expression of proteome research information is an important part. And stable isotope labeled quantitative method [1], the non-labeled protein is a semi-quantitative method of relying solely on mass spectrometry to compensate for the lack of signal strength made quantitative quantitative methods. This method combines the traditional chromatography in chromatographic peak area based quantitative approach to peptide peaks identified as the basis for selection. The advantages: good