Hepatitis B virus infection on pregnant women and intrauterine infection.docVIP

 PAGE \* MERGEFORMAT 6 Hepatitis B virus infection on pregnant women and intrauterine infection Of: CHANG Wang Wenguang Liu Guangqin [Keywords:] pregnant women infected with hepatitis B virus intrauterine infection Infants of hepatitis B virus (HBV) in the susceptible population, and mother to child transmission is an important route of transmission [1]. By enzyme-linked immunosorbent assay (ELISA) detection of serum HBV markers in pregnant women, while using fluorescence quantitative polymerase chain reaction (FQ PCR) technique with different HBV infection in pregnant women and cord blood serum HBV DNA testing, a combination of these two correlation analysis were made to explore the maternal HBV infection status of mother to child transmission of HBV. Subjects and methods 1. Object: February 2005 ~ February 2007 in our hospital antenatal clinic screening of hepatitis B virus surface antigen (HBsAg)-positive pregnant women for the study of 75 cases, including HBsAg, hepatitis B virus e antigen (HBeAg) , hepatitis B virus core antibody (HBcAb) were positive (referred to as HBeAg) in pregnant women, 45 cases, HBsAg, hepatitis B virus e antibody (HBeAb), HBcAb were positive (the three positive) and 30 cases of pregnant women .2 group of pregnant women all the normal liver function. Maternal venous blood collected before delivery, separation of serum analytes stored at -20 ℃. 2. Cord blood collection: (1) acquisition method. Newborns after delivery, the umbilical from the round about 5 ~ 10 cm with a hemostatic clamp lived at both ends of the umbilical cord, cut the umbilical cord between the two clamps, one end of the maternal side of the umbilical cord Choose thick and exposed in the umbilical vein, 75% ethanol disinfection, sterile vacuum blood placenta toward the direction of the needle puncture specimens from 5 ml of blood, well marked for inspection. (2) Note. blood collection personnel specially implementation of trained nurses cord blood