Hepatitis B virus X gene sequence phylogenetic tree analysis.docVIP

 PAGE \* MERGEFORMAT 11 Hepatitis B virus X gene sequence phylogenetic tree analysis Author: Hao Zhang, Gui-Ju Sun, Qian Geng-Sun, Tu Hong [Abstract] Objective: To examine the use of hepatitis B virus X gene region sequences to build the phylogenetic tree analysis of the reliability of genotype. Methods: NCBI gene bank from the United States to download HBV genome sequences were 249, of which 166 are known genotype sequences, 83 of unknown type sequence. The use of software and TreeView software ClustalX1.8 genotype X-region sequences are known to construct evolutionary tree diagrams, analysis of the genotypes obtained by this method is consistent with the original, and genotype analysis of the unknown-type sequences, and then S District Evolutionary tree analysis to be verified. Results: The genotype of the 166 has a clear sequence of X-gene region of sequence analysis of the genotypes to be fully in line with the original genotype. X-Zone and S sequence analysis method 83 unknown-type sequence analysis of the results are consistent, respectively A-16, B-17, C-type 27, D-21 and F-2, no found that E, G, and H-type. Conclusion: The use of hepatitis B virus X gene region sequences of genotype analysis is completely reliable, and helps the pathogenic mechanism of HBV. [Keywords:] Hepatitis B virus; genotype; phylogenetic tree; X gene Hepatitis B virus (HBV) are hepatotropic DNA viruses Branch, with double-stranded DNA molecular structure, length of about 3.2kb. As the HBV reverse transcription with mRNA for the intermediate copy, copy the lack of proofreading enzymes in the process of base pairing error-prone, resulting in HBV gene mutation is very frequent. Although the serum HBV-based classification has been established for many years, but not deeply reflect the variation of HBV virus and its clinical significance is not great. HBV genotyping method to HBV genome sequence of nucleotide heterogeneity ≥ 8% for the different genotypes of the sub-type cri