Hepatitis C virus NS4B of the liver cells cmyc and ras protein expression.docVIP

 PAGE \* MERGEFORMAT 6 Hepatitis C virus NS4B of the liver cells cmyc and ras protein expression Author: Chen Xia Li Changping, Jian-Yu Xu, Chen Feng [Abstract] Objective To study the hepatitis C virus non-structural protein 4B (NS 4B) of the liver cells oncogene c  myc and ras protein expression to explore the mechanism of liver cancer in its possible role. Methods of liposome-mediated method, blank carrier PXCN2 and hepatitis C virus NS4B plasmid PCXN2  NS4B into Chang liver cells, and G418 selection for stable passage, RT  PCR were identified by plasmid successfully transfected cells hepatic inflow , immunocytochemistry to observe the cells of c  myc and ras expression. The results obtained with the G418 resistant Chang liver cells; blank control group and the blank vector was no c  myc expression, transfection of NS4B group of c  myc expression rate (21.3 ± 1.2)%, with the blank control group and the blank vector group There was a significant difference; blank control group and the blank vector group ras weak positive expression of transfected ras positive expression of NS4B group, and blank control group and the blank vector group difference was significant. Conclusion of hepatitis C virus NS4B can promote the cancer gene c  myc and ras expression and may be carcinogenic mechanism of hepatitis C virus play an important role. [Keywords:] Hepatitis C virus non-structural protein 4B c  myc; ras; G418 The Effect of HCV NS4B on Expressions of c  myc Protein and ras Protein in Hepatic Cells. Keywords:: Hepatitis C virus; Non  structural protein 4B; c  myc; ras; G418 Hepatitis C is a global epidemic of infectious diseases, it is estimated that the global hepatitis C virus (HCV) infected more than 100 million people. Primary hepatocellular carcinoma (HCC) is a serious threat to human health, cancer, and the incidence rate showed an upward trend year by year, HCV and HCC close, but the mechanism of HCV-induced liver cancer has not yet u