Hepatitis C virus core gene cloning vector pGEMHCV - core of the building.docVIP

 PAGE \* MERGEFORMAT 13 Hepatitis C virus core gene cloning vector pGEMHCV / core of the building [Abstract] Objective To construct hepatitis C virus core gene cloning vector pGEM-HCV/core, for further study of the external guide sequence (external guide sequence, EGS) to guide the level of RNase P in RNA blocks hepatitis C virus core gene expression in well pre-foundation. Methods with HCV full-length gene pBRTM/HCV1  301l plasmid was amplified in Escherichia coli JMl09; extracted pBRTM/HCV1  301l plasmid; from pBRTM/HCV1  301l plasmid was amplified by PCR in HCV core gene fragment and insert it pGEM  3Z cloning vector; to get the restructuring pGEM-HCV/core cloning vector; last EcoR I and Hind III double digestion identified HCV core gene cloning vector. Results pGEM-HCV/core cloning vector by restriction enzyme digestion, gel electrophoresis proved insert fragment with the HCV core gene correspond to the size; by DNA sequencing to prove their insertion sequence consistent with the HCV core gene sequences. Conclusion HCV core gene was successfully constructed a cloning vector pGEM-HCV/core. [Keywords:] HCV; core gene; carrier; EGS Abstract: Objective To construct the clone plasmid containing the core gene of HCV, preparing for the research of blockage of the expression of HCV core gene guided by EGS at the level of mRNA.Methods After expanding plasmid pBRTM / HCV1  3011 (containing the whole genome of HCV) through E. coli JM109. The core gene with pBRTM / HCV1  3011 is amplified with PCR as the template; The core segment was inserted into the clone plasmid pGEM  3Z. Plasmid pGEM-HCV/core was digested with EcoR I and Hind III. Results The PCR result of HCV core has correct size and sequences. Conclusion The clone plasmid pGEM  HCV / core has successfully been constructed. Keywords:: HCV; core gene; vector; EGS In the many gene fragments of HCV core protein gene of the virus nucleic acid with a package to maintain the shape of the function