Hemistepta the new separation of phenolic acids and identification of.docVIP

 PAGE \* MERGEFORMAT 6 Hemistepta the new separation of phenolic acids and identification of [Abstract] Objective To study the chemical composition of clay lyrata. Methods Hemistepta 95% of the whole plant ethanol extract of ethyl acetate extract parts of the chromatographic separation, the basis of spectral data and physical and chemical properties to determine the structure of all compounds. The results were isolated and identified two compounds were as follows: 8 - Carboxymethyl - hydroxy ethyl cinnamate (1) and 8 - carboxymethyl - hydroxy cinnamic acid methyl ester (2). Conclusion Compounds 1 and 2 are new compounds. [Keywords:] Hemistepta chemical composition of phenolic acids Hemistepta Hemistepta lyrata Bunge for the Asteraceae Hemistepta species, widely distributed across the country. [1] documented that the plant has heat-clearing and detoxifying, the role of blood stasis and swelling, clinical for the treatment of hemorrhoids fistula, carbuncle swollen boils, bleeding and fractures and other injuries. The authors have reported isolated from the flavonoids and lignans and other constituents [2 ~ 4]. Paper continue to report from its isolated parts of ethyl acetate extraction of the phenolic acids of the two new components: 8 - Carboxymethyl - hydroxy ethyl cinnamate (1) and 8 - carboxymethyl - hydroxy cinnamic acid methyl ester (2). Its structural formula shown in Figure 1. Figure 1 Structural formula of compounds 1,2 An apparatus and materials Medicine in 2003, taken from Jiujiang County, Jiangxi Province, through the forest in Jiujiang, Jiangxi Institute of Botany, Tan Ce-ming, a researcher identified as Hemistepta lyrata Bunge. Fisher-Johns-type micro-melting point instrument (temperature uncorrected), Perkin-Elmer 241 model polarimeter, Autospec-UltimaETOF mass spectrometer, INOVA-500 NMR instrument. Silica gel column chromatography, thin layer chromatography silica gel plates were products of Qingdao Marine Chemical Factory, Sephadex LH-20 f