High Performance Liquid Chromatography Chuanmutong in the content of stigmasterol.docVIP

 PAGE \* MERGEFORMAT 7 High Performance Liquid Chromatography Chuanmutong in the content of stigmasterol Author: Wei-Qi Xing-Bin Dong Xiaoping [Abstract] Objective To develop a high performance liquid chromatography (HPLC) determination Chuanmutong in stigmasterol content. Methods Diamonsil C18 column for the column (150 mm * 4.6 mm, 5 μ m), mobile phase was methanol - water (97:3), the detection wavelength of 210 nm, flow rate 1.0 ml * min-1, sample volume 5 μ l, column temperature was 30 ℃ . Results stigmasterol sample volume in the range of 1.835 ~ 18.35 μ g has good linear relationship, r = 0.999 9, recoveries were 101.0% ~ 103.6%, RSD of 0.7% ~ 2.1% (n = 3). Conclusion The method accuracy and repeatability and can be used Chuanmutong quality control. [Keywords:] high-performance liquid chromatography stigmasterol Chuanmutong ‘Chinese Pharmacopoeia ‘2005 edition reproduces Chuanmutong pass for wooden RANUNCULACEAE Clematis armandii Franch. Or hydrangea vine Clematis montana Buch.-Ham.’s Dry cane. Chuan Mu Tong with a heat diuretic, pass through the milk under the effect, attending edema, negative urine and milk by closing less embolism, is commonly used in Chinese herbal medicine [1]. ‘Chinese Pharmacopoeia’ requirements to oleanolic acid Chuanmutong TLC as a characteristic ingredient. In our previous studies, according to ‘the Chinese Pharmacopoeia’ method of experiment results show that the nine groups were non-Chuanmutong this component, but in thin-layer chromatography showed a co-purple spots [2]. After component separation, identification, from small stems of Akebia of the four compounds were isolated and their structures were identified as 9,12-Octadecadienoic acid (zz) -2,3-dihydroxypropyl ester, ergosterol, stigmasterol alcohol and β -sitosterol , and compared with the TLC pattern analysis shows that appear on the above-mentioned thin-layer chromatography spots of the common purple ergosterol, stigmasterol, β -sitosterol mixture. HPLC and TLC