High Performance Liquid Chromatography Danshensu Xinshu granules Sodium.docVIP

 PAGE \* MERGEFORMAT 7 High Performance Liquid Chromatography Danshensu Xinshu granules Sodium [Abstract] Objective: To establish a determination of diastolic particle concentration method. Methods: Shim  pack CLC  ODS (5 μ m, 4.6 mm * 150 mm) as analytical column, methanol-acetonitrile   triethylamine phosphate in water (4:3:93) as mobile phase, detection wavelength 281 nm, injection volume of 10 μ L. Using HPLC determination of Danshensu sodium. Results: Danshensu sodium in 0.26 μ g-0.62 μ g within the linear relationship: Y =-137054.2 (r = 0.9998), the average recovery was 97.32%, RSD = 1.66% (n = 5). Conclusion: The method is simple, reliable, good reproducibility and can be used as the product quality control methods. [Keywords:] Xin Shu particle; Salvia sodium / separation and extraction; chromatography, high pressure liquid Xin Shu particles from Salvia, 37, and other medicinal herbs Gynostemma extract obtained by the scientific method of compound preparation. Used in clinical coronary heart disease, hyperlipidemia and other diseases. Prescriptions Salvia is one of the monarch herb, with blood stasis and pain, promoting blood circulation, the role of pure heart Chufan [1]. Danshensu of Salvia contains exclusive ingredients, is a water-soluble phenolic acids, has anti-platelet aggregation reconciliation, the role of poly [2]. Using high performance liquid chromatography of the preparation of the monarch herb Salvia Danshensu sodium as quantitative indicators, in order to effectively control the product quality. An experimental material 1.1 Instrument Shimadzu LC  10ATVP liquid chromatography, in the north chromatography workstation, SPD  10AVP detector. 1.2 reagent methanol, acetonitrile HPLC pure, water re-distilled water. Others were of analytical grade. 1.3 Reference Standards Danshensu sodium reference substance by the Chinese biological products available (855  200202 determination of use). 2 Experimental methods and