High Performance Liquid Chromatography Determination of Compound Puhuang intestinal capsule of gallic acid.docVIP

 PAGE \* MERGEFORMAT 7 High Performance Liquid Chromatography Determination of Compound Puhuang intestinal capsule of gallic acid [Abstract] Objective: To establish a high-performance liquid chromatography compound Puhuang intestinal capsule of gallic acid. Methods: The analytical column was C18 column (200mm * 4.6mm, 5μ m); mobile phase was methanol: 0.1% phosphoric acid solution (ten ninety); detection wavelength of 273nm. Results: gallic acid in the 26 ~ 56μ g/ml range a good linear relationship (r2 = 0.9999), the average recovery was 98.02, RSD is 2.18%. Conclusion: The method is simple, fast and accurate. [Keywords:] high-performance liquid chromatography; compound Puhuang bowel capsule; gallic acid Compound Puhuang bowel capsule of the Liaoning Institute of Medical Biotechnology newly developed compound traditional Chinese medicine preparation, to natural medicine Puhuang based medicine, joint gallnut, Sophora and other Chinese herbs, were extracted and made of enteric-coated capsules, antibacterial anti-inflammatory, hemostatic, astringent intestines, antispasmodic, antidiarrheal effect. Major clinical for the treatment of ulcerative colitis. Gallic acid is a pharmaceutical intermediates, but also the main component of Galla chinensis, it can combine with proteins on the intestinal bacteria have a direct killing effect [1]. Gallic acid by HPLC methods include colorimetry [2], TLC [3] and HPLC [4] method. In this experiment, the Gallic gallic acid as an index, using the same HPLC method with the USP of this product for quality control. An instrument and reagent High-performance liquid chromatography: Shimadzu SCL-10Avp high-performance liquid chromatograph, SPD-10Avp diode array detector, LC-10ATVP chromatography pump; compound Puhuang bowel capsule from the Liaoning Medical Biotechnology Institute, gallic acid control products, purchased from Chinese medicine and biological products for the determination of use; methanol chromatography pure; wate