High Performance Liquid Chromatography Determination of flavonoids in semipinnata.docVIP

 PAGE \* MERGEFORMAT 11 High Performance Liquid Chromatography Determination of flavonoids in semipinnata Author: Gong Xian-ling, Zhi-Hong Chen, Nian-Ci Liang 【Abstract】 Objective To determine semipinnata of luteolin and apigenin content. Methods ODS  C18 analytical column (25 mm × 4.6 mm, 5 μm); mobile phase was methanol -0.4% phosphoric acid solution (1:1); detection wavelength of 345 nm, flow rate 1 ml * min-1, column temperature at 25 ℃. Results of luteolin linear range 0.02 ~ 0.4 μg, r = 0.999 9, the average recovery was 96.39% (RSD = 1.95%); apigenin linear range of 0.02 ~ 0.4 μg, r = 0.999 6, the average recovery was 98.10 % (RSD = 0.96%). Conclusion This method is simple, accurate, high sensitivity, good reproducibility can be used as half of the flag of the determination of two flavonoids. Keywords: semipinnata; flavonoids; high-performance liquid chromatography Analysis and Determination of Flavonoids in Pteris semipinnata L. by HPLC Abstract: ObjectiveTo analyze and determine the content of luteolin and apigenin in Pteris semipinnata L. by HPLC. MethodsThe HPLC system consisted of ODS-C18 column (25 mm × 4.6 mm, 5 μm), methanol: 0.4% sodium mixture as a mobile phase , detection wavelength at 345nm, 1 ml * min-1 of flow rate and column temperature at 25 ℃. ResultsThe linear range of luteolin and apigenin were 0.02 ~ 0.4 μg, r = 0.999 9 and 0.02 ~ 0.4 μg, r = 0.999 6 respectively , the recovery were 96.39% (RSD = 1.95%, n = 5) and 98.10% (RSD = 0.96%, n = 5) respectively.ConclusionThe method is simple, accurate, sensitive and reproducible. This method can be used for the quantitative determination of flavonoids in Pteris semipinnata L. Keywords:: Pteris semipinnata L.; Flavonoids; HPLC Pteris Pteris semipinnata L. flag for Pteridaceae Pteris species, widely distributed in Sichuan and the Yangtze River south of the provinces and autonomous regions, bitter, Xin, sexual cool, with evacuation cold, dampn