High Performance Liquid Chromatography Dysosma the content of podophyllotoxin.docVIP

 PAGE \* MERGEFORMAT 13 High Performance Liquid Chromatography Dysosma the content of podophyllotoxin 【Abstract】 Objective To establish a high-performance liquid chromatography of podophyllotoxin content in Dysosma method. Methods using high performance liquid chromatography column was Diamonsiltm C18 column; mobile phase: methanol -0.1 mol / L potassium dihydrogen phosphate solution (50:50); flow rate: 1.0 ml / min; detection wavelength: 290 nm . The results of podophyllotoxin in 0.047 69 ~ 0.476 9 mg / ml showed good linear relationship (r = 0.999 6), the average recovery was 99.94%, RSD = 1.71% (n = 5). Conclusions The high-performance liquid chromatography Dysosma in podophyllotoxin content, simple, accurate, specific and strong. Keywords: high-performance liquid chromatography versipellis podophyllotoxin Abstract: ObjectiveTo establish a method of determining Podophllotoxin in Dysosma versipellis by HPLC.MethodsHigh Performance Liquid Chromatography was performed Diamonsil C18. The chromatography conditions were as follows: methanol - 0.1mol / L monobasic potassium phosphate (50:50) as mobile phase, flow rate being 1.0ml/min and detecting wavelength was 290nm.ResultsA good linearity of Podophllotoxin was shown in range of 0.047 69 ~ 0.476 9 mg / ml (r = 0.999 6), the average recovery was 99.94% and RSD was 1.71% (n = 5). ConclusionThe method can supply evidence for the quality evaluation of Dysosma versipellis. It is simple and accurate. Keywords:: HPLC; Dysosma versipellis; Podophllotoxin Dysosma of Berberidaceae plant Dysosma Dysosma versipellis (Hance.) M. cheng (Podophyllum versipelle Hance.) Roots and rhizomes. A Qingrejiedu, phlegm Sanjie, expectorant swelling of the function. For the carbuncle swollen boils, M. scrofulaceum, sore throat, bruises, snake bites and so on. I had Dysosma traits, microstructure had conducted research [1]. In this study, using high performance liquid chromatography Dysosma podophyll