High Performance Liquid Chromatography Five Tigers Powder HSYA A content of.docVIP

 PAGE \* MERGEFORMAT 7 High Performance Liquid Chromatography Five Tigers Powder HSYA A content of [Abstract] Objective To establish the compound preparation Five Tigers Powder Safflower active ingredients by HPLC. Methods Agilent C18 (5 μ m, 250 mm * 4.60 mm) column; -0.4% phosphoric acid in methanol water (33:67) as mobile phase, flow rate of 1 ml * min-1, detection wavelength was 402 nm. Results HSYA linear in the range of 0.056 5 ~ 0.452 μ g (r = 0.999 4), average recovery (n = 6) of 99.15%, RSD was 4.51%. Conclusion The method is simple, feasible, reproducible, and can be used for the preparation of safflower Determination of the active ingredient. [Keywords:] Five Tigers scattered; HSYA; high performance liquid chromatography Abstract: ObjectiveTo establish the method for determination of effective components in Wuhu powder by RP-HPLC. MethodsThe contents of hydroxysafflor yellow A was determined by an HPLC system using following conditions: the column was Agilent C18 (5 μ m, 250 mm * 4.60 mm) , with CH3OH-0.4% H3PO4water solution (33:67) as mokile phase, the flow rate was 1.0 ml * min-1, the detection wavelength was at 402 nm. ResultsThe linear range of hydroxysafflor yellow A was 0.056 5 ~ 0.452 μ g ( r = 0.999 4); its average recovery were 99.15%, corresponding RSD were 4.51%. Conclusion The HPLC method is simple, accurate, highly selective and reproducible, thus it can be used as quality control of Compound Wuhu powder. Keywords:: Wuhu powder; Hydroxysafflor yellow A; HPLC Fab Five bulk is ‘Chinese Pharmacopoeia’ income contained in the variety, from the Chinese angelica, safflower, wind, Arisaema (system), and Angelica and other five Chinese herbal medicines, has a casual blood circulation silt, swelling analgesic effect, for bruises, congestion swelling and so on. Statutory standards [1] only reproduces the microscopic identification and Chinese angelica, Angelica of the TLC qualitative identification. No determination of the standard. The ex