High Performance Liquid Chromatography Galangal in 2-phenyl-n-heptane content of A.docVIP

 PAGE \* MERGEFORMAT 11 High Performance Liquid Chromatography Galangal in 2-phenyl-n-heptane content of A Author: Zhou change lotus, Jiang Jianping, Liu Fanfan, Suenning [Abstract] Objective To determine the active ingredients in Alpinia diphenyl-n-heptane A content. Method of high-performance liquid chromatography (HPLC) method; Elite, Hypersil ODS 25 μ m (4.6 mm * 250 mm); detection wavelength 210 nm; column temperature 30 ℃ ; mobile phase of acetonitrile -0.1% phosphoric acid water (40:60); flow rate 1 ml / min. The results of 2-phenyl-n-heptane A linear range of 0.45 ~ 3.6 μ g, r = 0.999 93, recovery of 99.62%, RSD 1.53%. Conclusion The method is simple, linear relationship was good, can be used as Galangal in 2-phenyl-heptane-A as a basis for separation and detection. [Keywords:] high-performance liquid chromatography; Galangal A 2-phenyl-n-heptane Abstract: ObjectiveThe purpose is to determine the content of 5 - hydroxyl -7 - (4 amp;quot;- hydroxyl -3amp;quot; - methoxyphenyl)-1-pheny-3-heptanone from Alpinia officinarum Hance. MethodsThe HPLC was used to determine the content of 5 - hydroxyl -7 - (4 amp;quot;- hydroxyl -3amp;quot; - methoxyphenyl)-1-pheny-3-heptanone from Alpinia officinarum Hance, the analysis was carried out on Elite Hypersil ODS 25 μ m (4.6mm * 250mm), the mobile phase was Acetonitrile-0.1% H3PO4 (40:60), flow-rate 1.0ml/min and wavelength was 210nm, the colum temperature was 30 ℃ . ResultsThe linear range of 5 - hydroxyl -7 - (4 amp;quot;- hydroxyl -3amp;quot; -- methoxyphenyl)-1-pheny-3-heptanone was 0.45 ~ 3.6 μ g and correlation (r) was 0.999 93, recovery was 99.62% and RSD was 1.53%. ConclusionThe method is simple and presents a good linear relationship, so it can be used to determine the content of 5-hydroxyl-7-(4 amp;quot;-hydroxyl-3amp;quot;-methoxyphenyl)-1-pheny-3-heptanone from Alpinia officinarum Hance. Keywords:: HPLC; Alpinia officinarum Hance; 5  hydroxyl  7 - (4 amp;quot; hydroxyl  3amp;quot;  methoxyph