High Performance Liquid Chromatography in Xinjiang Pomegranate husk content of gallic acid.docVIP

 PAGE \* MERGEFORMAT 12 High Performance Liquid Chromatography in Xinjiang Pomegranate husk content of gallic acid 【Abstract】 Objective To determine the Xinjiang Pomegranate husk of gallic acid content. Methods using high performance liquid chromatography, Techsphere ODS (250 mm × 4.6 mm) column, mobile phase was methanol -0.05% phosphoric acid (five ninety-five), the detection wavelength was 273 nm. The results of gallic acid in 9 ~ 90 μg / ml within the linear, r = 0.999 7; the average recovery was 100.2%, RSD = 1.4% (n = 5). Conclusion The method is precise and reliable, can be used as quality control of medicinal POMEGRANATE PEEL approach. Keywords: Pomegranate husk of gallic acid in high-performance liquid chromatography Abstract: ObjectiveTo determine the content of gallic acid in Xinjiang Punica granatum L.by HPLC.MethodsTechsphere ODS (250 mm × 4.6 mm) was used. The mobile phase was methanol-0.05% H3PO4 (5:95). The detection wavelength was at 273nm.ResultsThe linear range of gallic acid was 9 ~ 90 μg / ml, r = 0.999 7.The average recovery was 100.1%, RSD = 1.4% (n = 5). ConclusionThis method is precise and credible, it can apply to the quality control of Punica granatum L. Keywords:: Punica granatium L.; Gallic acid; HPLC Pomegranate husk of pomegranate pomegranate punica grantum L. plants of the peel, Pomegranate husk of acid, astringent taste, with astringent intestinal bleeding, deworming and other effects. Pomegranate husk rich in tannins, modern research shows that Pomegranate husk tannins with spermicide [1], antibacterial [2], antiviral [3] effect, with the prospects for further development and application. Pomegranate husk of gallic acid as one of the active ingredient, it can be an integral part of tannin hydrolysis can lead to tumor cell growth inhibition or apoptosis [4], but also has strong anti-oxidation [5]. In this paper, high-performance liquid chromatography of the Pomegranate husk produced in the