Hepatocellular carcinoma in patients with abnormal methylation of RASSF1A significance of detection.docVIP

 PAGE \* MERGEFORMAT 7 Hepatocellular carcinoma in patients with abnormal methylation of RASSF1A significance of detection Authors: Zhao Qiang son, Qizhi Fang, Wei Wan-li, Li Kai-Zong, Ke-Feng Dou [Keywords:] liver neoplasms; RASSF1A gene; methylation; plasma Diagnostic value of detection of aberrantly methylated RASSF1A in plasma of patients with hepatocellular carcinoma [Abstract] AIM: To study the aberrant methylation status of Ras association domain family 1A (RASSF1A) in the plasma of patients with hepatocellular carcinoma (HCC) by methylationspecific PCR (MSP), and to explore the value of circular tumorrelated DNA in diagnosis of HCC. METHODS: Free DNA was extracted from peripheral blood plasma of 41 HCC patients. After modified with sodium bisulfite and purified, the DNA was amplified using primers specific for methylated and unmethylated sequences of RASSF1A. RESULTS: Aberrant RASSF1A methylation was found in 41.5% (17 of 41) of plasma DNA in HCC patients. No methylated RASSF1A was detected in 10 cases of control plasma samples. Clinical data including age, sex, clinical stages, levels of AFP, hepatitis status, number of tumor, invasion, metastasis and cirrhosis showed no correlation with aberrant methylation of RASSF1A (Pamp;gt; 0.05). Aberrantly methylated RASSF1A was also detected in 7 of 10 plasma samples of AFP negative cases. CONCLUSION: Detection of aberrant methylation of RASSF1A in blood is feasible to early detect and screen HCC , and is useful to estimate the prognosis. [Keywords] liver neoplasms; Ras association domain family 1A; methylation; plasma [Abstract] Objective: The methylation-specific PCR method (MSP) detected in 41 cases of primary hepatocellular carcinoma (HCC) in patients with plasma free Ras-related domain family protein 1A (RASSF1A) abnormally high methylation, and explore the plasma RASSF1A detection in the diagnosis of hepatocellular carcinoma. Methods: The plasma was extracted free DNA, sodium bisulfite-modified and