High Performance Liquid Chromatography heat Cough Capsules content of baicalin.docVIP

 PAGE \* MERGEFORMAT 11 High Performance Liquid Chromatography heat Cough Capsules content of baicalin Keywords: thermal Kechuan capsule;,, high-performance liquid chromatography;,, baicalin Abstract: Objective To establish a high-performance liquid chromatography thermal Cough Capsules content of baicalin. Methods 18 alkyl silane bonded silica gel as a filler; with methanol: water: phosphoric acid (47:53:0.2) as mobile phase; detection wavelength of 280 nm. The average recovery was 99.88%, RSD = 1.66%. Conclusion The detection method is simple and accurate and can be used for the preparation of baicalin were determined. Keywords:: thermal Kechuan capsules; high-performance liquid chromatography; baicalin Hot Kechuan capsules made preparations for my hospital variety, the whole party from the skullcap, ephedra, Tianzhu Huang and ground-lung composed of 17 Chinese herbal medicines, with heat phlegm, relieving cough and asthma the role of induced sputum for the heat cough, expectoration, wheezing embolism; and acute bronchitis, chronic bronchitis, acute exacerbation of symptoms of those who see the above. Of the original standards were not established assay methods to enhance the quality of the preparation of the controllability, we refer to the literature [1], using HPLC determination of baicalin baicalin content, and methodology of the determination methods for inspection, has made with satisfactory results. An instrument and reagent High-performance liquid chromatography: LC10ATvp pump, SPD10Avp UV detector, CTO10ASvp oven (Shimadzu); Shimadzu Libror AEL 40SM (one hundred thousandth) balance (Shimadzu, Japan); methanol (HPLC pure, Tianjin City-mao Chemical Reagent Factory); water (re-distillation, the Provisional prepared before use); phosphoric acid (AR, Tianjin Chemical Reagent Factory Damao); baicalin reference substance (in the testing laboratory, batch number: 110,715,200,212, for Determination of use); hot Kechuan