High Performance Liquid Chromatography Isodon clover content of oleanolic acid.docVIP

 PAGE \* MERGEFORMAT 12 High Performance Liquid Chromatography Isodon clover content of oleanolic acid Keywords: leaf Isodon;,, oleanolic acid;,, high-performance liquid chromatography Abstract: Objective To establish a high-performance liquid chromatography Isodon clover content of oleanolic acid. Method column ZORBAX XDBC18 (4.6 mm × 250 mm, 5 μm), mobile phase: methanol: water (90:10), detection wavelength 215 nm, flow rate 1.0 ml / min, column temperature: 30 ℃. Results oleanolic acid linear range of 0.462 ~ 4.62 μg (r = 0.999 5), recovery was 100.25%, RSD was 1.35%. Conclusion The method is simple, accurate, rapid and can be used for leaf Isodon of oleanolic acid in quality control. Keywords:: trefoil Isodon; oleanolic acid; high-performance liquid chromatography Determination of Oleanolic Acid in Elsholtzia lychnitis Levl. By HPLC Abstract: ObjectiveTo establish an HPLC method for the determination of Oleanolic acid in Elsholtzia lychnitis Levl .. MethodsColumn: ZORBAX XDBC18 (4.6 mm × 250 mm, 5 μm); mobile phase: methanol: water (90:10); detection wavelength was at 215 nm; temperature was at 30 ℃; flow rate was 1.0 ml / min. ResultsThe calibration curve showed a linearity with the range of 0.462 ~ 4.62 μg.The average recovery was 100.25% and RSD was 1.35%. ConclusionThe method is convenient, fast and reliable and suitable for the quality control of Elsholtzia lychnitis Levl.. Keywords:: Elsholtzia lychnitis Levl.; Oleanolic acid; HPLC Trefoil Isodon of Labiatae Isodon Elsholtzia lychnitis Levl. Of the whole plant, also known as three sisters, cavities medicine, Shelter grass; distributed in Guangxi, Guangdong, Guizhou, Yunnan and other places, its bitter taste micro-Xin a cool nature, “Chinese Medicine Dictionary” records that he has a Qufeng cold-dispelling, phlegm of the effectiveness of the wet grounds, attending cold cough, jaundice, rheumatism, swelling and so on. WANG Xuefen et al [1 ~ 3] were isolate