High Performance Liquid Chromatography Kudan grass capsule contents gentiopicroside.docVIP

 PAGE \* MERGEFORMAT 8 High Performance Liquid Chromatography Kudan grass capsule contents gentiopicroside [Abstract] Objective To establish a grass Kudan gentiopicrin capsule assay method. Methods using high performance liquid chromatography column: Dumas C18 column, methanol - water (30:70) as mobile phase, detection wavelength of 270 nm. Results gentiopicroside at 0.049 72 ~ 1.491 6μ g linear relationship between the good, r = 0.999 98, recovery was 97. 7%, RSD of 1.3%. Conclusion The method is simple and good separation, high sensitivity can be used as Kudan grass capsule quality control standards. [Keywords:] high-performance liquid chromatography Kudan grass capsules Gentiopicrin Grass Grass Capsules Kudan Kudan-chip [1,2] The formulations to change the variety, from the gentian, has a heat-clearing and dampness, diarrhea and other liver and gallbladder fire effects [3], for the damp-heat jaundice, swollen Yin Yin itching, vaginal discharge, Strong, the eczema itching, Mu Chi, deafness, Xie Tong, mouth pain, convulsion convulsion. The main active ingredient of gentian gentiopicroside [2]. To control product quality, to ensure efficacy, this paper, high-performance liquid chromatography gentian gentiopicroside content, with a view to the preparation of quality control for reference. An instrument and reagent LC-2010C high-performance liquid chromatography, LC-2010C UV detector, gentiopicroside (Chinese medicines and biological products 110770-200409 determination purposes), and methanol (HPLC pure, Tianjin Concord Technology Co., Ltd.), re-distilled water, and other reagents for the AR, Kudan grass capsules (Kang Chen, Shenyang Pharmaceutical Co., Ltd.). 2 Methods and Results 2.1 Preparation of Solution 2.1.1 Preparation of reference substance solution, said precision reference substance obtained gentiopicroside 12.43 mg, buy 50 ml Liang Ping, add methanol dissolved and diluted to the scale, shake. Precise volume of 5 ml to take home 25 ml Liang