High Performance Liquid Chromatography Kangfu Capsules matrine matrine oxide.docVIP

 PAGE \* MERGEFORMAT 15 High Performance Liquid Chromatography Kangfu Capsules matrine matrine oxide 【Abstract】 Objective: To establish Kang Capsule Women method for the determination. Methods: The high-performance liquid chromatography. Column: Agilent Technologies ZORBAX Extend-C18 4.6 × 250mm, 5μm, mobile phase: acetonitrile - methanol - phosphate buffer (PH6.8) (16: 16:68), flow rate: 1.0ml * min-1 The detection wavelength: 220nm, column temperature: 30 ℃. Results: matrine at 0.02 ~ 0.40mg * ml-1 range of a good linear relationship (r = 0.9995); The average recovery was 98.2%, RSD = 1.4% (n = 6); oxidation of matrine at 0.01 ~ 0.20mg * ml-1 range of a good linear relationship (r = 0.9997); The average recovery was 97.6%, RSD = 2.2% (n = 6). Conclusion: This method is simple, accurate and reliable, suitable for women Kang Capsule quality control. Keywords: high-performance liquid chromatography Kangfu Capsules Determination of matrine oxymatrine Determination of Matrine Oxgmatrine content in Kangfuling capsules by HPLC 【Abstract objective】 To set up a method for quality control of Fuyankang tablets. METHOD: HPLC method was developed to quantitative determination. The separation was performed on Agilent Technologies ZORBAX Extend-C18 4.6 × 250mm, 5μm.The mobile phase was acetonitrile-methanol -phosphate buffer (pH = 6.8) (16:16:68). The flow rate was 1.0ml * min-1. The UV detection wavelength was 220nm. The column temperature was 30 ℃. RESULTS: The linear range of Matrine and Oxgmatrine were at 0.02 ~ 0.40 mg * ml-1 (r = 0.9995) and 0.01 ~ 0.20 mg * ml-1 (r = 0.9997) respectively, the average recoveries (n = 6) were 98.2% and 97.6% with RSD 1.4% and 2.2%. CONCLUSION The method is simple and accurate, it can be used for quality control of Kangfuling capsules. 【Keywords:】 HPLC; Kangfuling capsules; Matrine; Oxgmatrine; Determination Kang Ling Capsule women as “the State Drug Administration Standard (Trial)” collec